Product composition: Preparation: A 2.0 mL collection tube, a 1.5 mL centrifuge tube, RNase A enzyme, suspension P1, lysis buffer P2, neutralization buffer P3, washing buffer W1, washing buffer W2, elution buffer E
Product presentation: This kit utilizes an optimized alkaline lysis method that breaks bacterial cells under alkaline conditions to separate plasmid DNA from chromosomal DNA. Plasmid DNA retains its supercoiled structure after neutralization, while chromosomal DNA precipitates. The precipitate is centrifuged to remove residual material, leaving plasmid DNA in the supernatant. Purification yields high-purity plasmid DNA. It is suitable for molecular biology experiments including sequencing, in vitro transcription/translation, restriction enzyme digestion, and bacterial transformation.
